Best Practice in Microbiome Research

This open access volume details protocols for sampling and analysis microbiomes from sites other than the gut. Chapters detail methods for analysing the virome, mycobiome, and emphasises the importance for inclusive and integrative microbiome studies.

Authoritative and cutting-edge, Best Practice in Microbiome Research aims to highlight critical concepts, key features, and serves as a valuable benchmark.

Inhalt

Research Ethics.- Study Design Considerations.- Collection, Storing and Processing of Fecal Samples for Human Microbiome Research.- Collection, Storing and Processing of Other Samples.- DNA Extraction and Quality Control.- 16S rRNA Library Preparation and Sequencing.- Shotgun Metagenomic Library Preparation.- Long Read Metagenomic Sequencing.- Reproducible and Scalable Bioinformatics.- Bioinformatics processing of 16S Datasets.- Bioinformatics processing of whole metagenome shotgun datasets.- Statistical Analysis of Microbiome Data.- Profiling microbial metabolites in human urine, fecal, and in vitro colon model samples.- Virome Analysis.- Mycobiomics.